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it binds with TERF1 bound to precoated antibody
Calculate the concentration of ANXA6 in the sample by plotting standard curve
Then a biotinylated detection antibody specific for Human OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human ESM1 in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Human SOD3 Antibody is added and binds to SOD3 in the sample
Mouse TRACP-5b (TartRate-Resistant Acid Phosphatase 5b) ELISA Kit CD40LG it binds with TERF1 boundThe Mouse TRACP 5b (TartRate Resistant Acid Phosphatase 5b) ELISA Kit measures TRACP 5b in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TRACP 5b antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components.
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