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Substrate solution is then added and color develops in proportion to the amount of Equine IL1B
The OD value is proportional to the concentration of Human D1R/DRD1
Then Streptavidin-HRP is added and binds to the Biotinylated NT-3 antibody
Only those wells that contain Human Beta Manase
The OD value is proportional to the concentration of Human PTPRK
Human IL-5 (Interleukin 5) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human IL 5 (Interleukin 5) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human IL 5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL 5 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
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